Alternative transcription start sites contribute to acute-stress-induced transcriptome response in human skeletal muscle.

ABSTRACT:

Background

More than half of human protein-coding genes have an alternative transcription start site (TSS). We aimed to investigate the contribution of alternative TSSs to the acute-stress-induced transcriptome response in human tissue (skeletal muscle) using the cap analysis of gene expression approach. TSSs were examined at baseline and during recovery after acute stress (a cycling exercise).

Results

We identified 44,680 CAGE TSS clusters (including 3764 first defined) belonging to 12,268 genes and annotated for the first time 290 TSSs belonging to 163 genes. The transcriptome dynamically changes during the first hours after acute stress; the change in the expression of 10% of genes was associated with the activation of alternative TSSs, indicating differential TSSs usage.

SUBMITTER: Makhnovskii PA 

PROVIDER: S-EPMC9308330 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature