Identification of clinical implications and potential prognostic models of chromatin regulator mutations in multiple myeloma.

ABSTRACT:

Background

With the rapid development of next-generation sequencing (NGS) technologies, researchers are making efforts to reveal the genomic landscape of multiple myeloma (MM). However, the clinical significance of many mutations remains poorly defined due to the genetic heterogeneity of MM. To systematically explore the clinical implications of gene mutations and build practical prognostic models, we performed DNA sequencing in newly diagnosed MM patients.

Methods

MM cells were purified from bone marrow aspirates using CD138 microbeads and subjected to sequencing with a 387-gene Panel. Nomogram was developed using Cox's proportional hazards model, and candidate variables were screened by stepwise regression. Internal validation was carried out by the bootstrap method.

Re

SUBMITTER: Zhang L 

PROVIDER: S-EPMC9308335 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature