Piezo1-Regulated Mechanotransduction Controls Flow-Activated Lymphatic Expansion.

ABSTRACT:

Background

Mutations in PIEZO1 (Piezo type mechanosensitive ion channel component 1) cause human lymphatic malformations. We have previously uncovered an ORAI1 (ORAI calcium release-activated calcium modulator 1)-mediated mechanotransduction pathway that triggers lymphatic sprouting through Notch downregulation in response to fluid flow. However, the identity of its upstream mechanosensor remains unknown. This study aimed to identify and characterize the molecular sensor that translates the flow-mediated external signal to the Orai1-regulated lymphatic expansion.

Methods

Various mutant mouse models, cellular, biochemical, and molecular biology tools, and a mouse tail lymphedema model were employed to elucidate the role of Piezo1 in flow-induced lymphatic growth and regenerat

SUBMITTER: Choi D 

PROVIDER: S-EPMC9308715 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature