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Data for crystallisation, dissolution and saturation temperatures of a model fuel comprising eicosane crystallising from supersaturated toluene solutions in the presence of a cold-flow improver additive.

ABSTRACT: The data presented in this article relates to the crystallisation of the long chain hydrocarbon eicosane (C₂₀H₄₂), from supersaturated toluene solutions in the absence/presence of a commercially available cold-flow improver additive (IA) at different solution treat rates. Data was collected for treat rates of 0, 0.1, 0.5, 2, 3, 5 and 10 wt% IA with respect to eicosane, with each treat rate studied over four solution concentrations. Data is collected by transmission vs. solution temperature experimental investigations and is analysed through a conventional transmission analysis route (STR) and a reanalysed route that takes into account multiple phase transformation behaviour (RRT). Average crystallisation and dissolution data is provided over a range of solution concentrations and cooling rates used under a polythermal crystallisation methodology for each analysis route. Equilibrium saturation temperature, supersolubility and metastable zone width data is also presented for each treat rate, concentration and analysis route. Laser transmission as a function of solution temperature profiles are displayed for IA crystallising from toluene solutions. This data relates to the research article: Kaskiewicz, P. L., Downie, R., Dowding, P. J., George, N. & Roberts, K. J. Influence of a Polymeric Additive on the Crystallisability and Nucleation Mechanism for the Model Fuel System of Eicosane Crystallising from Supersaturated Toluene Solutions. J. Cryst. Growth 581, (2021) 126,470. https://doi.org/10.1016/j.jcrysgro.2021.126470.

SUBMITTER: Kaskiewicz PL

PROVIDER: S-EPMC9309423 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Data for crystallisation, dissolution and saturation temperatures of a model fuel comprising eicosane crystallising from supersaturated toluene solutions in the presence of a cold-flow improver additive.

Kaskiewicz Peter L PL Downie Ruth R Dowding Peter J PJ George Neil N Roberts Kevin J KJ

Data in brief 20220708

The data presented in this article relates to the crystallisation of the long chain hydrocarbon eicosane (C<sub>20</sub>H<sub>42</sub>), from supersaturated toluene solutions in the absence/presence of a commercially available cold-flow improver additive (IA) at different solution treat rates. Data was collected for treat rates of 0, 0.1, 0.5, 2, 3, 5 and 10 wt% IA with respect to eicosane, with each treat rate studied over four solution concentrations. Data is collected by transmission vs. solu ...[more]

PMID: 35898860

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Project description:Directed evolution of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 previously created the hydroxylase alpha-subunit (TomA3) V106A variant (TOM-Green) with increased activity for both trichloroethylene degradation (twofold enhancement) and naphthalene oxidation (six-times-higher activity). In the present study, saturation mutagenesis was performed at position A106 with Escherichia coli TG1/pBS(Kan)TOMV106A to improve TOM activity for both chloroform degradation and naphthalene oxidation. Whole cells expressing the A106E variant had two times better naphthalene-to-1-naphthol activity than the wild-type cells (V(max) of 9.3 versus 4.5 nmol.min(-1).mg of protein(-1) and unchanged K(m)), and the regiospecificity of the A106E variant was unchanged, with 98% 1-naphthol formed, as was confirmed with high-pressure liquid chromatography. The A106E variant degrades its natural substrate toluene 63% faster than wild-type TOM does (2.12 +/- 0.07 versus 1.30 +/- 0.06 nmol.min(-1).mg of protein(-1) [mean +/- standard deviation]) at 91 microM and has a substantial decrease in regiospecificity, since o-cresol (50%), m-cresol (25%), and p-cresol (25%) are formed, in contrast to the 98% o-cresol formed by wild-type TOM. The A106E variant also has an elevated expression level compared to that of wild-type TOM, as evidenced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Another variant, the A106F variant, has 2.8-times-better chloroform degradation activity based on gas chromatography (V(max) of 2.61 versus 0.95 nmol.min(-1).mg of protein(-1) and unchanged K(m)) and chloride release (0.034 +/- 0.002 versus 0.012 +/- 0.001 nmol.min(-1).mg of protein(-1)). The A106F variant also was expressed at levels similar to those of wild-type TOM and 62%-better toluene oxidation activity than wild-type TOM (2.11 +/- 0.3 versus 1.30 +/- 0.06 nmol.min(-1).mg of protein(-1)). A shift in regiospecificity of toluene hydroxylation was also observed for the A106F variant, with o-cresol (28%), m-cresol (18%), and p-cresol (54%) being formed. Statistical analysis was used to estimate that 292 colonies must be screened for a 99% probability that all 64 codons were sampled during saturation mutagenesis.

Dataset Information

Data for crystallisation, dissolution and saturation temperatures of a model fuel comprising eicosane crystallising from supersaturated toluene solutions in the presence of a cold-flow improver additive.

Publications

Data for crystallisation, dissolution and saturation temperatures of a model fuel comprising eicosane crystallising from supersaturated toluene solutions in the presence of a cold-flow improver additive.

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