Redefining the N-Terminal Regulatory Region of the Ca²⁺/H⁺ Antiporter CAX1 in Tomato.

ABSTRACT: Calcium (Ca²⁺) is an essential plant nutrient, and Ca²⁺/H⁺ exchangers (CAXs) regulate Ca²⁺ partitioning between subcellular compartments. AtCAX1 activity is inhibited by its N-terminal regulatory region (NRR), which was initially defined as the sequence between the first two methionines. However, the accuracy of this NRR definition and the NRR regulatory mechanism remain unclear. Here, using tomato SlCAX1 as a model, we redefined the NRR of CAXs and demonstrated that our new definition is also applicable to Arabidopsis AtCAX1 and AtCAX3. The N-terminal-truncated SlCAX1 (SlCAX1^Δ39) but not the full-length SlCAX1 was active in yeast, similar to Arabidopsis AtCAX1. Characterization of slcax1 mutants generated by CRISPR-Cas9 confirm

SUBMITTER: Han B 

PROVIDER: S-EPMC9310016 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature