Defining mitochondrial protein functions through deep multiomic profiling.

ABSTRACT: Mitochondria are epicentres of eukaryotic metabolism and bioenergetics. Pioneering efforts in recent decades have established the core protein componentry of these organelles¹ and have linked their dysfunction to more than 150 distinct disorders^2,3. Still, hundreds of mitochondrial proteins lack clear functions⁴, and the underlying genetic basis for approximately 40% of mitochondrial disorders remains unresolved⁵. Here, to establish a more complete functional compendium of human mitochondrial proteins, we profiled more than 200 CRISPR-mediated HAP1 cell knockout lines using mass spectrometry-based multiomics analyses. This effort generated approximately 8.3 million distinct biomolecule measurements, providing a deep survey of the cellular respons

SUBMITTER: Rensvold JW 

PROVIDER: S-EPMC9310563 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature