Alternative LIM homeodomain splice variants are dynamically regulated at key developmental steps in vertebrates.

ABSTRACT:

Background

Alternative splicing provides a broad strategy to amplify the genome. Yet how alternative splicing influences neurodevelopment or indeed which variants are translated at developmental choice points remains poorly explored. Here we focused on a gene important for neurodevelopment, the Lim homeodomain transcription factor, Lhx9. Lhx9 has two noncanonical splice variants, Lhx9a and Lhx9b which compared with the canonical variant Lhx9c have a truncated homeodomain and an alternative C-terminal sequence, suggesting that, if translated, these variants could differently impact on cellular function.

Results

We created a unique antibody tool designed to selectively detect noncanonical Lhx9 variants (Lhx9ab) and used this to examine the protein expression dynamics in embryo

SUBMITTER: Wheaton BJ 

PROVIDER: S-EPMC9310833 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature