Revealing the Microbiome of Four Different Thermal Springs in Turkey with Environmental DNA Metabarcoding.

ABSTRACT: One of the most significant challenges for detecting microbial life in thermal springs by conventional techniques such as culturing is these places' physicochemical (temperature, heavy metal content, pH, etc.) conditions. Data from several studies suggest that high-throughput DNA sequencing technologies can be used to perform more accurate and detailed microbiome analyses. The primary aim of this paper was to determine the microbiome in the thermal source by metabarcoding environmental DNA isolated from four different sources and reveal the reflection of differences caused by temperature and chemical content on the microbiome. DNA was extracted from water filtered with enclosed filters and using the Illumina high-throughput sequencing platform, V3 and V4 regions of the 16S rRNA gene were s

SUBMITTER: Celik I 

PROVIDER: S-EPMC9311576 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature