Direct coupling of size exclusion chromatography and mass spectrometry for the characterization of complex monoclonal antibody products.

ABSTRACT: The present study describes the possibilities offered by an innovative bioinert size exclusion chromatography column for size variant characterization of complex monoclonal antibody products. This size exclusion chromatography column includes a novel column hardware surface. The column was prepared from metallic hardware components that were treated to have prototype hydrophilically modified hybrid organic-inorganic silica surfaces called hybrid surface technology. This provides a significant reduction in nondesired hydrophobic and electrostatic interactions that can occur between column and analyte when performing size exclusion chromatography analysis with volatile mobile phase. Compared to a reference stainless-steel column packed with the same batch of packing material, peak tailing, b

SUBMITTER: Murisier A 

PROVIDER: S-EPMC9311719 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature