Neuronal Depolarization Induced RNA m⁵C Methylation Changes in Mouse Cortical Neurons.

ABSTRACT: Neuronal activity is accomplished via substantial changes in gene expression, which may be accompanied by post-transcriptional modifications including RNA cytosine-5 methylation (m⁵C). Despite several reports on the transcriptome profiling of activated neurons, the dynamics of neuronal mRNA m⁵C modification in response to environmental stimuli has not been explored. Here, we provide transcriptome-wide maps of m⁵C modification, together with gene expression profiles, for mouse cortical neurons at 0 h, 2 h, and 6 h upon membrane depolarization. Thousands of differentially expressed genes (DEGs) were identified during the neuronal depolarization process. In stimulated neurons, the majority of early response genes were found to serve as expression regulators of

SUBMITTER: Xu X 

PROVIDER: S-EPMC9311806 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature