The Complete Genome of the "Flavescence Doree" Phytoplasma Reveals Characteristics of Low Genome Plasticity.

ABSTRACT: Members of the genus 'Candidatus Phytoplasma' are obligate intracellular bacteria restricted to phloem sieve elements and are able to colonize several tissues and the hemolymph in their insect vectors. The current unfeasibility of axenic culture and the low complexity of genomic sequences are obstacles in assembling complete chromosomes. Here, a method combining pathogen DNA enrichment from infected insects and dual deep-sequencing technologies was used to obtain the complete genome of a phytoplasma causing Grapevine Flavescence dorée. The de novo assembly generated a circular chromosome of 654,223 bp containing 506 protein-coding genes. Quality assessment of the draft showed a high degree of completeness. Comparative analysis with other phytoplasmas revealed the absence of potentia

SUBMITTER: Debonneville C 

PROVIDER: S-EPMC9312162 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature