Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept.

ABSTRACT: A new sensing platform based on long-period fiber gratings (LPFGs) for direct, fast, and selective detection of human immunoglobulin G (IgG; Mw = 150 KDa) was developed and characterized. The transducer's high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs) design for IgG detection. The sensing scheme is based on differential refractometric measurements, including a correction system based on a non-imprinted polymer (NIP)-coated LPFG, allowing reliable and more sensitive measurements, improving the rejection of false positives in around 30%. The molecular imprinted binding sites were performed on the surface of a LPFG with a sensitivity of about 130 nm/RIU and a FOM of 16 RIU^-1. The low-cost and easy to build device was tested in a work

SUBMITTER: Mendes JP 

PROVIDER: S-EPMC9312733 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature