Re-Programing Glucose Catabolism in the Microalga Chlorella sorokiniana under Light Condition.

ABSTRACT: The microalga Chlorella sorokiniana has attracted much attention for lipid production and wastewater treatment. It can perform photosynthesis and organic carbon utilization concurrently. To understand its phototrophic metabolism, a biomass compositional analysis, a ¹³C metabolic flux analysis, and metabolite pool size analyses were performed. Under dark condition, the oxidative pentose phosphate pathway (OPP) was the major route for glucose catabolism (88% carbon flux) and a cyclic OPP-glycolytic route for glucose catabolism was formed. Under light condition, fluxes in the glucose catabolism, tricarboxylic acid (TCA) cycle, and anaplerotic reaction (CO₂ fixation via phosphoenolpyruvate carboxylase) were all suppressed. Meanwhile, the RuBisCO reaction became act

SUBMITTER: Li T 

PROVIDER: S-EPMC9313030 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature