Characteristics of Normalization Methods in Quantitative Urinary Metabolomics-Implications for Epidemiological Applications and Interpretations.

ABSTRACT: A systematic comparison is presented for the effects of seven different normalization schemes in quantitative urinary metabolomics. Morning spot urine samples were analyzed with nuclear magnetic resonance (NMR) spectroscopy from a population-based group of 994 individuals. Forty-four metabolites were quantified and the metabolite-metabolite associations and the associations of metabolite concentrations with two representative clinical measures, body mass index and mean arterial pressure, were analyzed. Distinct differences were observed when comparing the effects of normalization for the intra-urine metabolite associations with those for the clinical associations. The metabolite-metabolite associations show quite complex patterns of similarities and dissimilarities between the different no

SUBMITTER: Li T 

PROVIDER: S-EPMC9313036 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature