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Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor.

ABSTRACT: Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host-guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.

SUBMITTER: Villalonga A

PROVIDER: S-EPMC9313153 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor.

Villalonga Anabel A Parrado Concepción C Díaz Raúl R Sánchez Alfredo A Mayol Beatriz B Martínez-Ruíz Paloma P Vilela Diana D Villalonga Reynaldo R

Biosensors 20220712 7

Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host-guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon elect ...[more]

PMID: 35884317

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