Adhesion of Gastric Cancer Cells to the Enteric Nervous System: Comparison between the Intestinal Type and Diffuse Type of Gastric Cancer.

ABSTRACT:

Background

Gastric cancer (GC) is the third leading cause of cancer-related deaths worldwide. The enteric nervous system (ENS) has been suggested to be involved in cancer development and spread.

Objective

To analyze the GC cell adhesion to the ENS in a model of co-culture of gastric ENS with GC cells.

Methods

Primary culture of gastric ENS (pcgENS), derived from a rat embryo stomach, was developed. The adhesion of GC cells to pcgENS was studied using a co-culture model. The role of N-Cadherin, a cell-adhesion protein, was evaluated.

Results

Compared to intestinal-type GC cells, the diffuse-type GC cancer cells showed higher adhesion to pcgENS (55.9% ± 1.075 vs. 38.9% ± 0.6611, respectively, p < 0.001). The number of diffuse-type GC cells adherent to p

SUBMITTER: Girot P 

PROVIDER: S-EPMC9313246 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature