Tracking Clonal Evolution of Multiple Myeloma Using Targeted Next-Generation DNA Sequencing.

ABSTRACT: Clonal evolution drives treatment failure in multiple myeloma (MM). Here, we used a custom 372-gene panel to track genetic changes occurring during MM progression at different stages of the disease. A tumor-only targeted next-generation DNA sequencing was performed on 69 samples sequentially collected from 30 MM patients. The MAPK/ERK pathway was mostly affected with KRAS mutated in 47% of patients. Acquisition and loss of mutations were observed in 63% and 37% of patients, respectively. Four different patterns of mutation evolution were found: branching-, mutation acquisition-, mutation loss- and a stable mutational pathway. Better response to anti-myeloma therapy was more frequently observed in patients who followed the mutation loss-compared to the mutation acquisition pathway. More tha

SUBMITTER: Salomon-Perzynski A 

PROVIDER: S-EPMC9313382 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature