A Specific and Sensitive Aptamer-Based Digital PCR Chip for Salmonella typhimurium Detection.

ABSTRACT: Food poisoning and infectious diseases caused by Salmonella typhimurium (S. typhimurium) are serious public health concerns for human health and food safety. The diversity and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of S. typhimurium in food samples. A method capable of identification, detection, and quantification of S. typhimurium is essential for addressing these issues. In this study, aptamer-coated magnetic beads (Apt-MBs) are employed as capture bio-probes to specifically and selectively concentrate S. typhimurium in food samples. A self-priming chip-based digital PCR was then presented as another biosensor for on-site detection and quantification of S. typhimurium cells. The chip we developed

SUBMITTER: Suo Y 

PROVIDER: S-EPMC9313467 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature