Human Mesenchymal Stem Cell-Derived Miniature Joint System for Disease Modeling and Drug Testing.

ABSTRACT: Diseases of the knee joint such as osteoarthritis (OA) affect all joint elements. An in vitro human cell-derived microphysiological system capable of simulating intraarticular tissue crosstalk is desirable for studying etiologies/pathogenesis of joint diseases and testing potential therapeutics. Herein, a human mesenchymal stem cell-derived miniature joint system (miniJoint) is generated, in which engineered osteochondral complex, synovial-like fibrous tissue, and adipose tissue are integrated into a microfluidics-enabled bioreactor. This novel design facilitates different tissues communicating while still maintaining their respective phenotypes. The miniJoint exhibits physiologically relevant changes when exposed to interleukin-1β mediated inflammation, which are similar to observations i

SUBMITTER: Li Z 

PROVIDER: S-EPMC9313499 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature