Imaging Net Retrograde Axonal Transport In Vivo: A Physiological Biomarker.

ABSTRACT:

Objective

The objective of this study is to develop a novel method for monitoring the integrity of motor neurons in vivo by quantifying net retrograde axonal transport.

Methods

The method uses single photon emission computed tomography to quantify retrograde transport to spinal cord of tetanus toxin fragment C (¹²⁵ I-TTC) following intramuscular injection. We characterized the transport profiles in 3 transgenic mouse models carrying amyotrophic lateral sclerosis (ALS)-associated genes, aging mice, and SOD1^G93A transgenic mice following CRISPR/Cas9 gene editing. Lastly, we studied the effect of prior immunization of tetanus toxoid on the transport profile of TTC.

Results

This technique defines a quantitative profile of net retrograde axonal transp

SUBMITTER: Lee PJ 

PROVIDER: S-EPMC9313858 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature