Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry.

ABSTRACT:

Background

The rapid urease test (RUT) is a major diagnostic tool for detecting Helicobacter pylori infection. This study aimed to establish an objective method for measuring the color changes in the RUT kit to improve the test's diagnostic accuracy.

Methods

A UV-visible spectrophotometer was selected as the colorimeter; experiments were conducted in three stages to objectively identify the color changes in the RUT kit.

Results

First, the urea broth solution showed an identifiable color change from yellow to red as the pH increased by 0.2. The largest transmittance difference detected using the UV-visible spectrophotometer was observed at a 590-nm wavelength. Second, the commercialized RUT kit also showed a gradual color change according to the pH change detect

SUBMITTER: Pih GY 

PROVIDER: S-EPMC9313973 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature