Postmitotic differentiation of human monocytes requires cohesin-structured chromatin.

ABSTRACT: Cohesin is a major structural component of mammalian genomes and is required to maintain loop structures. While acute depletion in short-term culture models suggests a limited importance of cohesin for steady-state transcriptional circuits, long-term studies are hampered by essential functions of cohesin during replication. Here, we study genome architecture in a postmitotic differentiation setting, the differentiation of human blood monocytes (MO). We profile and compare epigenetic, transcriptome and 3D conformation landscapes during MO differentiation (either into dendritic cells or macrophages) across the genome and detect numerous architectural changes, ranging from higher level compartments down to chromatin loops. Changes in loop structures correlate with cohesin-binding, as well as

SUBMITTER: Minderjahn J 

PROVIDER: S-EPMC9314343 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature