Agro-waste as a substrate for the production of pullulanase by Penicillium viridicatum under solid-state fermentation.

ABSTRACT: One of the key enzymes utilized in the food industry is pullulanase. But its major drawbacks are its low yield and high production costs. In this regard, the current research aims to screen agro-waste substrates for optimal pullulanase production in solid-state fermentation. Of various agro-wastes used as a substrate, the maximum enzymic activity (9.74 U/gds) was observed in a medium based on 5 g of green gram husk and incubated for 3 days at 30 °C. The effects of 16 different nutrients on the yield of pullulanase production were studied using the Plackett-Burman experimental design. The incorporation of FeSO₄, MnSO₄, and MgSO₄ into the pullulanase production medium significantly increased the yield and showed a 5.7-fold increase (56.25 U/gds) in comparison

SUBMITTER: Kumar V 

PROVIDER: S-EPMC9314376 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature