In vitro efficacy of a non-instrumentation technique to remove intracanal multispecies biofilm.

ABSTRACT:

Aim

The aim of this study was to assess the efficacy of a non-instrumentation technique to disinfect root canals infected by a human dental plaque-derived multispecies biofilm.

Methodology

Twenty-two mandibular incisors were accessed, autoclaved and inoculated with dental plaque. The Center for Disease Control biofilm reactor was used to promote contamination of the root canal space. In the conventional technique (control), the specimens were instrumented until size 35/04 and irrigated with 6% NaOCl. In the non-instrumentation technique, a glide path was established using K-files size 10-20 and specimens were immediately cleaned with the GentleWave System. Samples were obtained for culture and 16S rRNA gene sequencing. Differences in abundances of genera were evaluated using

SUBMITTER: Ordinola-Zapata R 

PROVIDER: S-EPMC9314631 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature