Physiology-Based Pharmacokinetic Study on 18β-Glycyrrhetic Acid Mono-Glucuronide (GAMG) Prior to Glycyrrhizin in Rats.

ABSTRACT: To understand that 18β-Glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) showed better pharmacological activity and drug-like properties than 18β-Glycyrrhizin (GL); a rapid and sensitive HPLC-MS/MS method was established for the simultaneous determination of GAMG and its metabolite 18β-Glycyrrhetinic acid (GA) in rat plasma and tissues after oral administration of GAMG or GL. This analytical method was validated by linearity, LLOQ, specificity, recovery rate, matrix effect, etc. After oral administration, GAMG exhibited excellent C_max (2377.57 ng/mL), T_max (5 min) and AUC_0-T (6625.54 mg/L*h), which was much higher than the C_max (346.03 ng/mL), T_max (2.00 h) and AUC_0-T (459.32 mg/L*h) of GL.

SUBMITTER: Cao M 

PROVIDER: S-EPMC9315563 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature