Fine Mapping and Gene Analysis of restorer-of-fertility Gene CaRf_HZ in Pepper (Capsicum annuum L.).

ABSTRACT: Cytoplasmic male sterility (CMS) is a common biological phenomenon used in hybrid production of peppers (Capsicum annuum L.). Although several restorer-of-fertility (Rf) genes of pepper CMS lines have been mapped, there is no report that the Rf gene with clear gene function has been isolated. Here, pepper CMS line HZ1A and its restorer line HZ1C were used to construct (HZ1A × HZ1C) F₂ populations and map the Rf gene. A single dominant gene CaRfHZ conferred male fertility according to inheritance analysis. Using sterile plants from (HZ1A × HZ1C) F₂ populations and bulked segregant analysis (BSA), the CaRf_HZ gene was mapped between P06gInDel-66 and P06gInDel-89 on chromosome 6. This region spans 533.81 kb, wher

SUBMITTER: Nie Z 

PROVIDER: S-EPMC9316182 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature