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Laser-Induced Breakdown Spectroscopy Associated with the Design of Experiments and Machine Learning for Discrimination of Brachiaria brizantha Seed Vigor.

ABSTRACT: Laser-induced breakdown spectroscopy (LIBS) associated with machine learning algorithms (ML) was used to evaluate the Brachiaria seed physiological quality by discriminating the high and low vigor seeds. A 23 factorial design was used to optimize the LIBS experimental parameters for spectral analysis. A total of 120 samples from two distinct cultivars of Brachiaria brizantha seeds exhibiting high vigor (HV) and low vigor (LV) in standard tests were studied. The raw LIBS spectra were normalized and submitted to outlier verification, previously to the reduction data dimensionality from principal component analysis. Supervised machine learning algorithm parameters were chosen by leave-one-out cross-validation in the test samples, and it was tested by external validation using a new set of data. The overall accuracy in external validation achieved 100% for HV and LV discrimination, regardless of the cultivar or the classification algorithm.

SUBMITTER: Cioccia G 

PROVIDER: S-EPMC9316187 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Laser-Induced Breakdown Spectroscopy Associated with the Design of Experiments and Machine Learning for Discrimination of <i>Brachiaria brizantha</i> Seed Vigor.

Cioccia Guilherme G   Pereira de Morais Carla C   Babos Diego Victor DV   Milori Débora Marcondes Bastos Pereira DMBP   Alves Charline Z CZ   Cena Cícero C   Nicolodelli Gustavo G   Marangoni Bruno S BS  

Sensors (Basel, Switzerland) 20220706 14

Laser-induced breakdown spectroscopy (LIBS) associated with machine learning algorithms (ML) was used to evaluate the <i>Brachiaria</i> seed physiological quality by discriminating the high and low vigor seeds. A 2<sup>3</sup> factorial design was used to optimize the LIBS experimental parameters for spectral analysis. A total of 120 samples from two distinct cultivars of <i>Brachiaria brizantha</i> seeds exhibiting high vigor (HV) and low vigor (LV) in standard tests were studied. The raw LIBS  ...[more]

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