Diagnostic application of transcripts associated with antibody-mediated rejection in kidney transplant biopsies.

ABSTRACT:

Background

The diagnosis of antibody-mediated rejection (AMR) is reached using the Banff Classification for Allograft Pathology, which now includes gene expression analysis. In this study, we investigate the application of 'increased expression of thoroughly validated gene transcripts/classifiers strongly associated with AMR' as diagnostic criteria.

Method

We used quantitative real-time polymerase chain reaction for 10 genes associated with AMR in a retrospective cohort of 297 transplant biopsies, including biopsies that met the full diagnostic criteria for AMR, even without molecular data (AMR, n = 27), biopsies that showed features of AMR, but that would only meet criteria for AMR with increased transcripts [suspicious for AMR (AMRsusp), n = 49] and biopsies that would nev

SUBMITTER: Toulza F 

PROVIDER: S-EPMC9317169 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature