Efficient Identification of the MYC Regulator with the Use of the CRISPR Library and Context-Matched Database Screenings.

ABSTRACT: MYC is a major oncogene that plays an important role in cell proliferation in human cancers. Therefore, the mechanism behind MYC regulation is a viable therapeutic target for the treatment of cancer. Comprehensive and efficient screening of MYC regulators is needed, and we had previously established a promoter screening system using fluorescent proteins and the CRISPR library. For the efficient identification of candidate genes, a database was used, for which mRNA expression was correlated with MYC using datasets featuring "Similar" and "Not exactly similar" contexts. INTS14 and ERI2 were identified using datasets featuring the "Similar" context group, and INTS14 and ERI2 were capable of enhancing MYC promoter activity. In furt

SUBMITTER: Tanaka Y 

PROVIDER: S-EPMC9317319 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature