Overexpression of an Inositol Phosphorylceramide Glucuronosyltransferase Gene IbIPUT1 Inhibits Na⁺ Uptake in Sweet Potato Roots.

ABSTRACT: IPUT1 is a glycosyltransferase capable of synthesizing the glycosyl inositol phosphorylceramide (GIPC) sphingolipid. The GIPC sphingolipid is a Na⁺ receptor on cell membranes which can sense extracellular Na⁺ concentrations, promote the increase in intracellular Ca²⁺ concentrations, and plays critical roles in maintaining intracellular Na⁺ balance. Therefore, the IPUT1 gene plays an important role in the genetic improvement of crop salt tolerance. Herein, the IbIPUT1 gene, which encodes an ortholog of Arabidopsis AtIPUT1, from sweet potato was cloned. Agrobacterium rhizogenes-mediated in vivo transgenic technology, non-invasive micro-measuring technology (NMT) and Na⁺ fluorescence imaging technology w

SUBMITTER: Liu C 

PROVIDER: S-EPMC9317492 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature