Correlation analysis of serum reproductive hormones and metabolites during multiple ovulation in sheep.

ABSTRACT:

Background

The establishment of non-invasive diagnostic method for multiple ovulation prediction is helpful to improve the efficiency of multiple ovulation. The blood hormones and metabolites would be suitable indexes for this subject.

Methods

In this study, 86 estrus ewes (65 of induced estrus (IE) and 21 of spontaneous estrus (SE)) were selected and the blood samples were collected at the day before follicle-stimulating hormone (FSH) injection (1^st) and before artificial insemination (2^nd). The serum reproductive hormones ofFSH, luteinizing hormone (LH), 17β-Estradiol (E2), progesterone (P4) and anti-Mullerian hormone (AMH) were measured through enzyme linked immunosorbent assay (ELISA) and the untargeted metabolomics analysis was processed through LC

SUBMITTER: Xu Q 

PROVIDER: S-EPMC9317590 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature