Spatial and Functional Crosstalk between the Mitochondrial Na⁺-Ca²⁺ Exchanger NCLX and the Sarcoplasmic Reticulum Ca²⁺ Pump SERCA in Cardiomyocytes.

ABSTRACT: The mitochondrial Na⁺-Ca²⁺ exchanger, NCLX, was reported to supply Ca²⁺ to sarcoplasmic reticulum (SR)/endoplasmic reticulum, thereby modulating various cellular functions such as the rhythmicity of cardiomyocytes, and cellular Ca²⁺ signaling upon antigen receptor stimulation and chemotaxis in B lymphocytes; however, there is little information on the spatial relationships of NCLX with SR Ca²⁺ handling proteins, and their physiological impact. Here we examined the issue, focusing on the interaction of NCLX with an SR Ca²⁺ pump SERCA in cardiomyocytes. A bimolecular fluorescence complementation assay using HEK293 cells revealed that the exogenously expressed NCLX was localized in close proximity to four exogenously expressed

SUBMITTER: Takeuchi A 

PROVIDER: S-EPMC9317594 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature