Melanoma Stem Cells Educate Neutrophils to Support Cancer Progression.

ABSTRACT:

Background

It is now well-established that cancer stem cells (CSCs) can support melanoma progression by reshaping the tumor immune microenvironment. However, the molecular mechanisms underlying the crosstalk between melanoma SCs and cancer-associated neutrophils have not been elucidated yet.

Methods

The aim of the present study was to unravel the role of melanoma SCs in neutrophil polarization. HL60 neutrophil-like (dHL60) cells were treated with conditioned medium from A375 melanoma SCs (CSC-CM), and their phenotype was investigated.

Results

We demonstrated that CSC-CM could specifically activate immune cells by increasing CD66b and CD11b expression. In particular, we revealed that A375 CSCs could release various soluble factors, namely TGF-β, IL-6, and IL-8, able to

SUBMITTER: Anselmi M 

PROVIDER: S-EPMC9317939 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature