Impact of Chromosomal Context on Origin Selection and the Replication Program.

ABSTRACT: Eukaryotic DNA replication is regulated by conserved mechanisms that bring about a spatial and temporal organization in which distinct genomic domains are copied at characteristic times during S phase. Although this replication program has been closely linked with genome architecture, we still do not understand key aspects of how chromosomal context modulates the activity of replication origins. To address this question, we have exploited models that combine engineered genomic rearrangements with the unique replication programs of post-quiescence and pre-meiotic S phases. Our results demonstrate that large-scale inversions surprisingly do not affect cell proliferation and meiotic progression, despite inducing a restructuring of replication domains on each rearranged chromosome. Remarkably,

SUBMITTER: Lanteri L 

PROVIDER: S-EPMC9318681 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature