Dynamic Features of Chromosomal Instability during Culture of Induced Pluripotent Stem Cells.

ABSTRACT: Induced pluripotent stem cells (iPSCs) hold great potential for regenerative medicine. By reprogramming a patient's own cells, immunological rejection can be avoided during transplantation. For expansion and gene editing, iPSCs are grown in artificial culture for extended times. Culture affords potential danger for the accumulation of genetic aberrations. To study these, two induced pluripotent stem (iPS) cell lines were cultured and periodically analyzed using advanced optical mapping to detect and classify chromosome numerical and segmental changes that included deletions, insertions, balanced translocations and inversions. In one of the lines, a population trisomic for chromosome 12 gained dominance over a small number of passages. This appearance and dominance of the culture by chromos

SUBMITTER: DuBose CO 

PROVIDER: S-EPMC9318709 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature