Development of a Novel Peptide Nucleic Acid Probe for the Detection of Legionella spp. in Water Samples.

ABSTRACT: Legionella are opportunistic intracellular pathogens that are found throughout the environment. The Legionella contamination of water systems represents a serious social problem that can lead to severe diseases, which can manifest as both Pontiac fever and Legionnaires' disease (LD) infections. Fluorescence in situ hybridization using nucleic acid mimic probes (NAM-FISH) is a powerful and versatile technique for bacterial detection. By optimizing a peptide nucleic acid (PNA) sequence based on fluorescently selective binding to specific bacterial rRNA sequences, we established a new PNA-FISH method that has been successfully designed for the specific detection of the genus Legionella. The LEG22 PNA probe has shown great theoretical performance, presenting 99.9% specific

SUBMITTER: Nacher-Vazquez M 

PROVIDER: S-EPMC9318766 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature