Rapid, Sensitive, and Selective Quantification of Bacillus cereus Spores Using xMAP Technology.

ABSTRACT: Bacillus cereus is a spore-forming ubiquitous bacterium notable as a food poisoning agent. Detection of B. cereus spores using selective media is laborious and non-specific. Herein, the quantitative detection of B. cereus spores was investigated with commercial antibodies and published aptamer sequences. Several detection reagents were screened for affinity to Bacillus collagen-like protein A (BclA), an abundant exosporium glycoprotein. Sensitivity and selectivity toward B. cereus spores were tested using immunoassays and multi-analyte profiling (xMAP). A recombinant antibody developed in llama against BclA protein showed B. cereus spore selectivity and sensitivity between 10² and 10⁵ spores/mL using xMAP. DNA aptamer sequence

SUBMITTER: Moteshareie H 

PROVIDER: S-EPMC9319878 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature