Urine Cellular DNA Point Mutation and Methylation for Identifying Upper Tract Urinary Carcinoma.

ABSTRACT:

Background

To improve the selection of patients for ureteroscopy, avoid excessive testing and reduce costs, we aimed to develop and validate a diagnostic urine assay for upper tract urinary carcinoma (UTUC).

Methods

In this cohort study we recruited 402 patients from six Hunan hospitals who underwent ureteroscopy for hematuria, including 95 patients with UTUC and 307 patients with non-UTUC findings. Midstream morning urine samples were collected before ureteroscopy and surgery. DNA was extracted and qPCR was used to analyze mutations in TERT and FGFR3 and the methylation of NRN1. In the training set, the random forest algorithm was used to build an optimal panel. Lastly, the Beijing cohort (n = 76) was used to validate the panel.

Results

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SUBMITTER: Ouyang W 

PROVIDER: S-EPMC9319988 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature