High TRB3 expression induces chondrocyte autophagy and senescence in osteoarthritis cartilage.

ABSTRACT:

Objective

Osteoarthritis is closely related to aging. Tribbles homologue 3 (TRB3) is found to display age-related expression and contributes to the regulation of cell proliferation, differentiation and fibrosis. In this study, we aimed to investigate the potential involvement of TRB3 in cartilage autophagy and aging in osteoarthritis.

Methods

Cartilage tissue samples were collected from osteoarthritis patients who received joint replacement and cadaveric donors. In osteoarthritis cartilage tissue, we analyzed autophagy- and senescence-associated proteins using immunohistochemistry and western blot (WB), in vitro, to confirm the role played by TRB3 in the process of autophagy, cell senescence, and inflammation, small interfering RNA (siRNA) was used for TRB3 knockdown

SUBMITTER: Gu Y 

PROVIDER: S-EPMC9320551 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature