In vivo detection of carnosine and its derivatives using chemical exchange saturation transfer.

ABSTRACT:

Purpose

To detect carnosine, anserine and homocarnosine in vivo with chemical exchange saturation transfer (CEST) at 17.2 T.

Methods

CEST MR acquisitions were performed using a CEST-linescan sequence developed in-house and optimized for carnosine detection. In vivo CEST data were collected from three different regions of interest (the lower leg muscle, the olfactory bulb and the neocortex) of eight rats.

Results

The CEST effect for carnosine, anserine and homocarnosine was characterized in phantoms, demonstrating the possibility to separate individual contributions by employing high spectral resolution (0.005 ppm) and low CEST saturation power (0.15 μ$$ \mu $$ T). The CEST signature of these peptides was evidenced, in vivo, in the rat brain and skeletal muscle. The pr

SUBMITTER: Bardin S 

PROVIDER: S-EPMC9320878 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature