Identification of Exported Plasmodium falciparum Proteins That Bind to the Erythrocyte Cytoskeleton.

ABSTRACT: Plasmodium proteins are exported to the erythrocyte cytoplasm to create an environment that supports parasite replication. Although hundreds of proteins are predicted to be exported through Plasmodium export element (PEXEL)-dependent and -independent mechanisms, the functions of exported proteins are largely uncharacterized. In this study, we used a biochemical screening approach to identify putative exported P. falciparum proteins that bound to inside-out vesicles prepared from erythrocytes. Out of 69 P. falciparum PEXEL-motif proteins tested, 18 bound to inside-out vesicles (IOVs) in two or more independent assays. Using co-affinity purifications followed by mass spectrometry, pairwise co-purification experiments, and the split-luciferase assay, we identified

SUBMITTER: Shakya B 

PROVIDER: S-EPMC9320996 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature