Precise Detection and Visualization of Nanoscale Temporal Confinement in Single-Molecule Tracking Analysis.

ABSTRACT: The plasma membrane consists of a diverse mixture of molecules that dynamically assemble into a highly non-random organization. The formation of nanoscale domains in the membrane is of particular interest as these domains underlie critical cellular functions. Single-molecule tracking is a powerful method to detect and quantify molecular motion at high temporal and spatial resolution and has therefore been instrumental in understanding mechanisms that underlie membrane organization. In single-molecule trajectories, regions of temporal confinement can be determined that might reveal interesting biophysical interactions important for domain formation. However, analytical methods for the detection of temporal confinement in single-molecule trajectories depend on a variety of parameters that he

SUBMITTER: Westra M 

PROVIDER: S-EPMC9320997 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature