Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of Enterotoxigenic <i>Escherichia coli</i> BE311 for Fluorescence Labeling and Enterotoxin Analyses.

ABSTRACT: Some prevention strategies, including vaccines and antibiotic alternatives, have been developed to reduce enterotoxigenic Escherichia coli proliferation in animal production. In this study, a wild-type strain of BE311 with a virulent heat-stable enterotoxin gene identical to E. coli K99 was isolated for its high potential for gene expression ability. The whole genome of E. coli BE311 was sequenced for gene analyses and editing. Subsequently, the fluorescent gene mCherry was successfully knocked into the genome of E. coli BE311 by CRISPR/Cas9. The E. coli BE311−mCherry strain was precisely quantified through the fluorescence intensity and red colony counting. The inflammatory factors in different intestinal tissues all increased significantly after an E. coli BE311−mCherry challenge in Spra

SUBMITTER: Lu S 

PROVIDER: S-EPMC9321511 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature