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Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study.

ABSTRACT: Paecilomyces spp. are emerging fungal pathogens, where Paecilomyceslilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents a species complex, whereas P. lilacinus is related to another genus called Purpureocillium. The aims of this study were to identify clinical isolates of Paecilomyces spp. at the species level, and to determine their antifungal susceptibility profiles. 70 clinical Paecilomyces spp. isolates were identified by MALDI-TOF Mass Spectrometry (MS) and by multilocus rDNA genes sequencing including ITS and the D1/D2 genes. Among the 70 Paecilomyces spp. isolates, 28 were identified as P. lilacinum, 26 as P. variotii stricto sensu, and 16 as P. maximus. For antifungal susceptibility testing, Minimal Inhibitory Concentrations (MICs) or Minimal Effective Concentrations (MECs) were determined for 8 antifungals. All P. lilacinum isolates had high MICs and MECs of amphotericin B and echinocandins, respectively, unlike P. variotii and P. maximus. For azole drugs, MICs were molecule- and species- dependent. The differences in in vitro susceptibility to antifungals underline the importance of accurate species identification. The MALDI-TOF MS can be a good alternative in routine laboratory to ensure fast identification of Paecilomyces spp. and P. lilacinum.

SUBMITTER: Monpierre L

PROVIDER: S-EPMC9321559 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study.

Monpierre Lorra L Aït-Ammar Nawel N Valsecchi Isabel I Normand Anne-Cécile AC Guitard Juliette J Riat Arnaud A Huguenin Antoine A Bonnal Christine C Sendid Boualem B Hasseine Lilia L Raberin Hélène H Dehais Marion M Ranque Stéphane S Hennequin Christophe C Piarroux Renaud R Dannaoui Eric E Botterel Françoise F

Journal of fungi (Basel, Switzerland) 20220629 7

Paecilomyces spp. are emerging fungal pathogens, where Paecilomyceslilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents a species complex, whereas P. lilacinus is related to another genus called Purpureocillium. The aims of this study were to identify clinical isolates of Paecilomyces spp. at the species level, and to determine their anti ...[more]

PMID: 35887446

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Dataset Information

Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study.

Publications

Species Identification and In Vitro Antifungal Susceptibility of <i>Paecilomyces</i>/<i>Purpureocillium</i> Species Isolated from Clinical Respiratory Samples: A Multicenter Study.

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