Fusion Tag Design Influences Soluble Recombinant Protein Production in Escherichia coli.

ABSTRACT: Fusion protein technologies to facilitate soluble expression, detection, or subsequent affinity purification in Escherichia coli are widely used but may also be associated with negative consequences. Although commonly employed solubility tags have a positive influence on titers, their large molecular mass inherently results in stochiometric losses of product yield. Furthermore, the introduction of affinity tags, especially the polyhistidine tag, has been associated with undesirable changes in expression levels. Fusion tags are also known to influence the functionality of the protein of interest due to conformational changes. Therefore, particularly for biopharmaceutical applications, the removal of the fusion tag is a requirement to ensure the safety and efficacy of the therapeutic

SUBMITTER: Koppl C 

PROVIDER: S-EPMC9321918 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature