Ontology highlight
ABSTRACT:
SUBMITTER: Anzai T
PROVIDER: S-EPMC9322095 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Anzai Takahiro T Saijou Shinji S Takashima Hiroki H Hara Misato M Hanaoka Shingo S Matsumura Yasuhiro Y Yasunaga Masahiro M
Pharmaceuticals (Basel, Switzerland) 20220706 7
Development of antibodies against the native structure of membrane proteins with multiple transmembrane domains is challenging because it is difficult to prepare antigens with native structures. Previously, we successfully developed a monoclonal antibody against multi-pass membrane protein TMEM180 by exosome immunization in rats. This approach yielded antibodies that recognized cancer-specific antigens on the exosome. In this study, we performed immunoprecipitation using magnetic beads to identi ...[more]