Unknown

Dataset Information

0

Identification of CD73 as the Antigen of an Antigen-Unknown Monoclonal Antibody Established by Exosome Immunization, and Its Antibody-Drug Conjugate Exerts an Antitumor Effect on Glioblastoma Cell Lines.


ABSTRACT: Development of antibodies against the native structure of membrane proteins with multiple transmembrane domains is challenging because it is difficult to prepare antigens with native structures. Previously, we successfully developed a monoclonal antibody against multi-pass membrane protein TMEM180 by exosome immunization in rats. This approach yielded antibodies that recognized cancer-specific antigens on the exosome. In this study, we performed immunoprecipitation using magnetic beads to identify the antigen of one of the rat antibody clones, 0614, as CD73. We then converted antibody 0614 to human chimeric antibody 0614-5. Glioblastoma (GB) was the cancer type with the highest expression of CD73 in the tumor relative to healthy tissue. An antibody-drug conjugate (ADC) of 0614-5 exerted an antitumor effect on GB cell lines according to expression of CD73. The 0614-5-ADC has potential to be used to treat cancers with high CD73 expression. In addition, our strategy could be used to determine the antigen of any antibody produced by exosome immunization, which may allow the antibody to advance to new antibody therapies.

SUBMITTER: Anzai T 

PROVIDER: S-EPMC9322095 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Identification of CD73 as the Antigen of an Antigen-Unknown Monoclonal Antibody Established by Exosome Immunization, and Its Antibody-Drug Conjugate Exerts an Antitumor Effect on Glioblastoma Cell Lines.

Anzai Takahiro T   Saijou Shinji S   Takashima Hiroki H   Hara Misato M   Hanaoka Shingo S   Matsumura Yasuhiro Y   Yasunaga Masahiro M  

Pharmaceuticals (Basel, Switzerland) 20220706 7


Development of antibodies against the native structure of membrane proteins with multiple transmembrane domains is challenging because it is difficult to prepare antigens with native structures. Previously, we successfully developed a monoclonal antibody against multi-pass membrane protein TMEM180 by exosome immunization in rats. This approach yielded antibodies that recognized cancer-specific antigens on the exosome. In this study, we performed immunoprecipitation using magnetic beads to identi  ...[more]

Similar Datasets

2024-09-13 | GSE241135 | GEO
| S-EPMC7640354 | biostudies-literature
2018-08-30 | GSE112752 | GEO
| S-EPMC10966576 | biostudies-literature
| S-EPMC5620149 | biostudies-literature
| S-EPMC9167454 | biostudies-literature
| S-EPMC11380396 | biostudies-literature
| S-EPMC5387159 | biostudies-literature
| S-EPMC8688850 | biostudies-literature
| S-EPMC6450958 | biostudies-literature