Advancing respiratory-cardiovascular physiology with the working heart-brainstem preparation over 25 years.

ABSTRACT: Twenty-five years ago, a new physiological preparation called the working heart-brainstem preparation (WHBP) was introduced with the claim it would provide a new platform allowing studies not possible before in cardiovascular, neuroendocrine, autonomic and respiratory research. Herein, we review some of the progress made with the WHBP, some advantages and disadvantages along with potential future applications, and provide photographs and technical drawings of all the customised equipment used for the preparation. Using mice or rats, the WHBP is an in situ experimental model that is perfused via an extracorporeal circuit benefitting from unprecedented surgical access, mechanical stability of the brain for whole cell recording and an uncompromised use of pharmacological agents akin to in vit

SUBMITTER: Paton JFR 

PROVIDER: S-EPMC9322470 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature