Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates.

ABSTRACT: Eukaryotic cells partition enzymes and other cellular components into distinct subcellular compartments to generate specialized biochemical niches. A subclass of these compartments form in the absence of lipid membranes, via liquid-liquid phase separation of proteins to form biomolecular condensates or "membraneless organelles" such as nucleoli, stress granules, and P-bodies. Because of their propensity to form compartments from simple starting materials, membraneless organelles are an attractive target for engineering new functionalities in both living cells and protocells. In this work, we demonstrate incorporation of a novel enzymatic activity in protein coacervates with the light-generating enzyme, NanoLuc, to produce bioluminescence. Using condensates comprised of the disordered RGG d

SUBMITTER: Guan M 

PROVIDER: S-EPMC9322593 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature