Thermodynamic and Kinetic Binding Behaviors of Human Serum Albumin to Silver Nanoparticles.

ABSTRACT: A nanoparticle, under biological milieu, is inclined to be combined with various biomolecules, particularly protein, generating an interfacial corona which provides a new biological identity. Herein, the binding interaction between silver nanoparticles (AgNPs) and human serum albumin (HSA) was studied with transmission electron microscopy (TEM), circular dichroism (CD), and multiple spectroscopic techniques. Due to the ground state complex formed mainly through hydrophobic interactions, the fluorescence titration method proved that intrinsic fluorescence for HSA was probably statically quenched by AgNPs. The complete thermodynamic parameters were derived, indicating that the interaction between HSA and AgNPs is an entropy-driven process. Additionally, synchronous fluorescence and CD spectr

SUBMITTER: Tian J 

PROVIDER: S-EPMC9323290 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature