A Computational-Experimental Investigation of the Molecular Mechanism of Interleukin-6-Piperine Interaction.

ABSTRACT: Herein, we elucidate the biophysical aspects of the interaction of an important protein, Interleukin-6 (IL6), which is involved in cytokine storm syndrome, with a natural product with anti-inflammatory activity, piperine. Despite the role of piperine in the inhibition of the transcriptional protein NF-κB pathway responsible for activation of IL6 gene expression, there are no studies to the best of our knowledge regarding the characterisation of the molecular interaction of the IL6-piperine complex. In this context, the characterisation was performed with spectroscopic experiments aided by molecular modelling. Fluorescence spectroscopy alongside van't Hoff analyses showed that the complexation event is a spontaneous process driven by non-specific interactions. Circular dichroism aided by mo

SUBMITTER: Povinelli APR 

PROVIDER: S-EPMC9323498 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature